Genetics.pdf
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Microsoft PowerPoint - Genet18.PPT
Homework:
12.1 through 12.8 (rest of ch. 12 problems
will not be on test).
EXAM WEDNESDAY, 11/6
Fig. 20.26 Structure of dideoxynucleotides
dNTP
1) ddNTPs have no 3’OH, terminate replication.
2) Uses: Anti-viral drugs (anti-HIV)
DNA sequencing
ddNTP
Fig. 20.27
DNA sequencing
DNA sequencing
reactions contain
template to be
sequenced, primer,
dNTPs, and one
ddNTP.
Some chains
terminate at bases
containing the
ddNTP.
Fig. 20.29 Results of modern automated
DNA sequencing.
Four reactions, each with a different ddNTP,
and each labeled with a different fluorescent
dye. Bands of same color=same base.
Fig. 11.13 Some DNA polymerase have other
enzyme activities. Ex. DNA pol I
5’—>3’ polymerase
5’—>3’ exonuclease
3’—5’ exonuclease
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michalak.daria
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