Bioanalytical Chemistry (Susan R. Mikkelsen & Eduardo Corton).pdf

BIOANALYTICAL

CHEMISTRY

BIOANALYTICAL

CHEMISTRY

Susan R. Mikkelsen

Eduardo Cort´ n

A JOHN WILEY & SONS, INC., PUBLICATION

Published by John Wiley & Sons, Inc., Hoboken, New Jersey.

Published simultaneously in Canada.

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Library of Congress Cataloging-in-Publication Data:

Mikkelsen, Susan R., 1960–

Bioanalytical chemistry / Susan R. Mikkelsen, Eduardo Cort´n.

p. cm.

Includes bibliographical references and index.

ISBN 0-471-54447-7 (cloth)

1. Analytical biochemistry.

[DNLM: 1. Chemistry, Analytical. QY 90 M637b 2004] I. Cort ´ n,

Eduardo, 1962– II. Title.

QP519.7.M54 2004

572 0 .36–dc22

2003016568

Printed in the United States of America

10987654321

CONTENTS

Preface

Acknowledgments

xvii

1. Spectroscopic Methods for Matrix Characterization

1.1 Introduction

1.2 Total Protein

1.2.1 Lowry Method

1.2.2 Smith (BCA) Method

1.2.3 Bradford Method

1.2.4 Ninhydrin-Based Assay

1.2.5 Other Protein Quantitation Methods

1.3 Total DNA

1.3.1 Diaminobenzoic Acid Method

1.3.2 Diphenylamine Method

1.3.3 Other Fluorometric Methods

1.4 Total RNA

1.5 Total Carbohydrate

1.5.1 Ferricyanide Method

1.5.2 Phenol–Sulfuric Acid Method

1.5.3 2-Aminothiophenol Method

1.5.4 Purpald Assay for Bacterial Polysaccharides

1.6 Free Fatty Acids

References

Problems

2. Enzymes

2.1 Introduction

2.2 Enzyme Nomenclature

2.3 Enzyme Commission Numbers

2.4 Enzymes in Bioanalytical Chemistry

2.5 Enzyme Kinetics

2.5.1 Simple One-Substrate Enzyme Kinetics

2.5.2 Experimental Determination of Michaelis–Menten

Parameters

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