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HPLC

A Practical Guide

RSC Chromatography Monographs

Series Editor: Roger M. Smith, Loughborough University,

Loughborough, UK

Advisory Panel: J.C. Berridge, Sandwich, UK; G.B. Cox, Illkirch, France;

I.S. Lurie, Virginia, USA; P.J. Schoenmaker, Amsterdam, The Netherlands;

C.F. Simpson, London, UE, G.G. Wallace, Wollongong, Australia.

This series is designed for the individual practising chromatographer, providing

guidance and advice on a wide range of chromatographic techniques with the

emphasis on important practical aspects of the subject.

Packed Column SFC

by T.A. Berger, Berger Instruments, Newark,

Delaware, USA

Chromatographic Integration Methods, Second Edition

by Norman Dyson, Dyson Instruments Ltd, UK

Separation of Fullerenes by Liquid Chromatography

edited by IS. Jinno, Toyohashi University of Technology, Japan

HPLC: A Practical Guide

by T. Hanai, Health Research Foundation, Kyoto, Japan

How to obtain future titles on publication

A standing order plan is available for this series. A standing order will bring delivery of each new

volume immediately upon publication. For further information, please write to:

The Royal Society of Chemistry, Turpin Distribution Services Ltd., Blackhorse Road, Letchworth,

Hertfordshire SG6 1 HN, UK.

Telephone: Letchworth +44(0) 1462 672555: Fax: + (44)O 1462 480947

CHROMATOGRAPHY

MONOGRAPHS

HPLC

A Practical Guide

T. Hanai

Health Research Foundation, Kyoto, Japan

RSC

ROYAL SOCIETY OF CHEMISTRY

ISBN 0-85404-515-5

A catalogue record for this book is available from the British Library

0 The Royal Society of Chemistry 1999

Apart from any fair dealing for the purposes of research or private study, or criticism or

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this publication may not be reproduced, stored or transmitted, in anyform or by any means,

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concerning reproduction outside the terms stated here should be sent to The Royal Society

of Chemistry at the address printed on this page.

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Cambridge CB4 OWF, UK

For further information see our web site at www.rsc.org

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Printed by Bookcraft (Bath) Ltd

Preface

high-performance liquid

be separated and on why

particular compounds are separated by liquid chromatographic methods. It

consists of six chapters: Basic Concepts of HPLC; Instrumentation: Prepara-

tion, Testing and Selectivity of Stationary Phase Materials; Selection of the

Eluent; Separation Based on an Improved Column Efficiency; Influence of

Physical Chemistry on Separations in Liquid Chromatography. The focus is on

the basic considerations in liquid chromatography rather than the applications

but there are also sections on trouble-shooting. The book is concerned mainly

with the selection of a packing material and the preparation of the eluent.

Theoretical optimization is demonstrated using the chromatography of simple

chemicals, to aid the understanding of liquid chromatography by graduate

students. The examples given go beyond what can be found in general textbooks

on liquid chromatography. The separations can be easily understood from the

differences in properties of familiar compounds known to undergraduate

students.

The chromatographic separations described are based on solubility using

molecular properties. The selection of chromatographic mode and column is

analysed using solubility parameters. The separation factor cc is described in

detail by physical and chemical parameters. Discussion of instrumentation

focuses on high-efficiency operation and is concerned with degassing, the time

constant, flow cell design, connectors and trouble-shooting. Packing materials

and their synthesis and surface modification, liquid chromatographic analysis

of surface activity and the evaluation of packed columns, including the

measurement of void volume, are also considered.

I thank Professor Roger Smith, of Loughborough University, who reviewed

the original manuscript and offered many helpful suggestions. I also deeply

appreciate the support of The Royal Society of Chemistry for this publication.

Toshihiko Hanai, Yokohama

This book answers two basic questions regarding

chromatography; it focuses on how compounds can

HPLC -A Practical Guide 1999 - Hanai.pdf

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