HPLC A practical user's guide 2ed 2007 - McMaster.pdf - eBooks - Tkanczak

HPLC

HPLC

A Practical User’s Guide

SECOND EDITION

Marvin C. McMaster

WILEY-INTERSCIENCE

A John Wiley & Sons, Inc., Publication

Published by John Wiley & Sons, Inc., Hoboken, New Jersey.

Published simultaneously in Canada.

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Library of Congress Cataloging-in-Publication Data:

McMaster, Marvin C.

HPLC, a practical user’s guide / Marvin C. McMaster. – 2nd ed.

p. cm.

Includes bibliographical references and index.

ISBN-13: 978-0-471-75401-5 (cloth)

ISBN-10: 0-471-75401-3 (cloth)

1. High performance liquid chromatography. I. Title.

QD79.C454M36 2007

543

2006040640

Printed in the United States of America.

10987654321

′

.84–dc22

CONTENTS

PREFACE

I HPLC PRIMER

1 Advantages and Disadvantages of HPLC

1.1 How It Works / 4

1.1.1 A Separation Model of the Column / 5

1.1.2 Basic Hardware: A Quick, First Look / 7

1.1.3 Use of Solvent Gradients / 8

1.1.4 Ranges of Compounds / 9

1.2 Other Ways to Make My Separation / 9

1.2.1 FPLC—Fast Protein Liquid Chromatography / 10

1.2.2 LC—Traditional Liquid Chromatography / 10

1.2.3 GLC—Gas Liquid Chromatography / 11

1.2.4 SFC—Supercritical Fluid Chromatography / 11

1.2.5 TLC—Thin Layer Chromatography / 12

1.2.6 EP—Electrophoresis / 12

1.2.7 CZE—Capillary Zone Electrophoresis / 13

2 Selecting an HPLC System

2.1 Characteristic Systems / 16

2.1.1 Finding a Fit: Detectors and Data Processing / 16

2.1.2 System Models: Gradient Versus Isocratic / 16

2.1.3 Vendor Selection / 17

2.1.4 Brand Names and Clones / 17

2.1.5 Hardware–Service–Support / 18

2.2 System Cost Estimates / 19

2.2.1 Type I System—QC Isocratic (Cost: $10–15,000) / 19

2.2.2 Type II System—Research Gradient

(Cost: $20–25,000) / 19

HPLC A practical user's guide 2ed 2007 - McMaster.pdf

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