Principles of Fluorescence Spectroscopy 3rd - Lakowicz - 9780387312781.pdf - Spektroskopia molekularna - hinatka3991

Principles of

Fluorescence Spectroscopy

Third Edition

Principles of

Fluorescence Spectroscopy

Third Edition

Joseph R. Lakowicz

University of Maryland School of Medicine

Baltimore, Maryland, USA

Joseph R. Lakowicz

Center for Fluorescence Spectroscopy

University of Maryland School of Medicine

Baltimore, MD 21201

USA

Library of Congress Control Number: 2006920796

ISBN-10: 0-387-31278-1

ISBN-13: 978-0387-31278-1

Printed on acid-free paper.

Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly

analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or

dissimilar methodology now known or hereafter developed is forbidden.

The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken

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Printed in Singapore.

(KYO)

987654321

springer.com

Dedicated to Mary,

for her continuous support and encouragement,

without whom this book would not have been written

Preface

The first edition of Principles was published in 1983, and

the second edition 16 years later in 1999. At that time I

thought the third edition would not be written until 2010 or

later. However, the technology of fluorescence has

advanced at an accelerating pace. Single-molecule detec-

tion and fluorescence-correlation spectroscopy are becom-

ing almost routine. New classes of probes have appeared,

such as the semiconductor nanoparticles, or QDots, and

genetically engineered green fluorescent probes. Addition-

ally, it is now becoming possible to control the excited

states of fluorophores, rather than relying only on sponta-

neous emission. These developments are changing the par-

adigm of fluorescence, from a reliance on organic fluo-

rophores, to the use of genetic engineering, nanotechnolo-

gy, and near-field optics.

I wish to express my appreciation and special thanks to

the individuals who have assisted me in the preparation of

the book. These include Ignacy Gryczynski for assistance

with the figures, Krystyna Gryczynski for drawing the fig-

ures, Joanna Malicka for proofreading the chapters, Kazik

Nowaczyk for the cover design and color digitizing of all

figures, Tim Oliver for typesetting, and the NIH for their

support of my laboratory. And finally, Mary, for her endless

hours of typing, correspondence and support.

Joseph R. Lakowicz

vii

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