glikoliza i cykl kwasu cytrynowego - enzymy.pdf

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MM03_ENZ.AI

GLYCOLYSIS & TCA CYCLE -

ENZYMES

HHOHH

HOCH 2 ---C---C---C---C--CHO

OH OH H OH

GLUCOSE

ATP

HHOHH

2.7.1.1

P-OCH 2 ---C---C---C---C--CHO

OH OH H OH

ADP

Glucose-6-P

HHOH

5.3.1.9

P-OCH 2

---C---C---C---CO--CH 2 OH

OH OH H

Fructose-6- P

ATP

HHOH

2.7.1.11

P-OCH 2

---C---C---C---CO--CH 2 OP

OH OH H

ADP

Fructose-1,6-bis-P

P- OC H 2

COCH 2 OH

Glycerone-3- P

4.1.2.13

(Di-OH-acetone- P)

P-OCH 2

CH(OH)CHO

3-P-Glyceraldehyde

2NAD +

1 . 2.1.12

2P i

P-OCH 2

CH(OH)COO-

P

2

NADH+2H

+

1,3-bis-P-Glycerate

2ADP

2.7.2.3

P -OCH 2 CH(OH)COO _

2

ATP

3-P-Glycerate

_

5.4.2.1

P-OCH 2 CH(O- )COO

P

2-P-Glycerate

_

4.2.1.11

CH 2 =C(O - P) COO

2H 2 O

P-enolpyruvate

2ADP

CH 3 COCOO _

2 .7.1.40

2ATP

PYRUVATE

PYRUVATE

2HSCoA

2NAD +

1.2.4.1

2CO 2

ACETYL-CoA

2NADH+2H +

2H 2 O

4.1.3.7

2HSCoA

- OOCCOCH 2 COO -

CH 2 COO -

C(OH)COO -

CH 2 COO -

Oxaloacetate

Citrate

4.2.1.3

1.1.1.37

2NAD +

2NADH+2H +

- OOCCH(OH)CH 2 COO -

CH(OH)COO -

CHCOO -

CH 2 COO -

Malate

Isocitrate

2H 2 O

2NAD +

4.2.1.2

2CO 2

1.1.1.41

- OOCCH=CHCOO -

- OOCCOCH 2 CH 2 COO -

2NADH+2H +

Fumarate

2-Oxoglutarate

2UQH 2

1.3.5.1

2UQ

2NAD +

- OOCCH 2 CH 2 COO -

1.2.4.2

2 CO 2

Succinate

- OOCCH 2 CH 2 CO.SCoA

2NADH+2H +

Succinyl-CoA

2P i

2GDP

6.2.1.4 3.1.2.3

2HSCoA

2GTP

ENZYMES

1.1.1.37 Malate dehydrogenase

1.1.1.41 Isocitrate dehydrogenas e

1.2.1.12 Glyceraldehyde-3-P dehydrogenase

1.2.4.1 Pyruvate dehydrogenase (lipoamide)

1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)

1.3.5.1 Succinate dehydrogenase (ubiquinone)

2.7.1.1 Hexokinase

2.7.1.11 6-Phosphofructokinase

3.1.2.3 Succinyl-CoA hydrolase

2.7.1.40 Pyruvate kinase

2.7.2.3 Phosphoglycerate kinase

4.1.2.13 Fructose-bis-phosphate aldolase

4.1.3.7 Citrate synthase

4.2.1.2 Fumarate hydratase

4.2.1.3 Aconitate hydratase

4.2.1.11 Phosphopyruvate hydratase

5.3.1.9 Glucose-6-phosphate isomerase

5.4.2.1 Phosphoglycerate mutase

6.2.1.4 Succinate-CoA ligase

The IUBMB Enzyme Commission (EC) Numbers are all 4-figure numbers, the first of which denotes

one of only 6 CLASSES of enzyme:

1... Oxidoreductases 2... Transferases 3...Hydrolases...4 Lyases...5...Isomerases and 6... Ligases

This identifies the biochemical nature of every reaction and is worth memorising

c 2001 IUBMB

CH 3 CO.SCoA

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