Genetics(1).pdf

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Microsoft PowerPoint - Genet18.PPT

Homework:

12.1 through 12.8 (rest of ch. 12 problems

will not be on test).

EXAM WEDNESDAY, 11/6

Fig. 20.26 Structure of dideoxynucleotides

dNTP

1) ddNTPs have no 3’OH, terminate replication.

2) Uses: Anti-viral drugs (anti-HIV)

DNA sequencing

ddNTP

Fig. 20.27

DNA sequencing

reactions contain

template to be

sequenced, primer,

dNTPs, and one

ddNTP.

Some chains

terminate at bases

containing the

ddNTP.

Fig. 20.29 Results of modern automated

DNA sequencing.

Four reactions, each with a different ddNTP,

and each labeled with a different fluorescent

dye. Bands of same color=same base.

Fig. 11.13 Some DNA polymerase have other

enzyme activities. Ex. DNA pol I

5’—>3’ polymerase

5’—>3’ exonuclease

3’—5’ exonuclease