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MolecularBiologyandBiotechnology
5thEdition
MolecularBiologyand
Biotechnology
5thEdition
Editedby
JohnMWalker
SchoolofLifeSciences,UniversityofHertfordshire,Hateld,Hertfordshire
AL109AB,UK
RalphRaply
SchoolofLifeSciences,UniversityofHertfordshire,Hateld,Hertfordshire
AL109AB,UK
ISBN:978-0-85404-125-1
AcataloguerecordforthisbookisavailablefromtheBritishLibrary
r
RoyalSocietyofChemistry,2009
Allrightsreserved
Apartfromfairdealingforthepurposesofresearchfornon-commercialpurposesorfor
privatestudy,criticismorreview,aspermittedundertheCopyright,DesignsandPatents
Act1988andtheCopyrightandRelatedRightsRegulations2003,thispublicationmaynot
bereproduced,storedortransmitted,inanyformorbyanymeans,withouttheprior
permissioninwritingofTheRoyalSocietyofChemistryorthecopyrightowner,orinthe
caseofreproductioninaccordancewiththetermsoflicencesissuedbytheCopyright
LicensingAgencyintheUK,orinaccordancewiththetermsofthelicencesissuedbythe
appropriateReproductionRightsOrganizationoutsidetheUK.Enquiriesconcerning
reproductionoutsidethetermsstatedhereshouldbesenttoTheRoyalSocietyofChemistry
attheaddressprintedonthispage.
PublishedbyTheRoyalSocietyofChemistry,
ThomasGrahamHouse,SciencePark,MiltonRoad,
CambridgeCB40WF,UK
RegisteredCharityNumber207890
Forfurtherinformationseeourwebsiteatwww.rsc.org
Preface
Oneoftheexcitingaspectsofbeinginvolvedintheeldofmolecular
biologyistheever-acceleratingrateofprogress,bothinthedevelop-
mentofnewmethodologiesandinthepracticalapplicationsofthese
methodologies.Indeed,suchdevelopmentsledtotheideaoftherst
editionofMolecularBiologyandBiotechnologyandsubsequentedi-
tionshavereectedthefast-movingnatureofthearea,notleastthis
latestedition,whichcontinuestoreectrecentdevelopments,with
newchaptersindevelopingareassuchasgenometechnology,nano-
biotechnology,regenerativemedicineandbiofuels.
Therstsixchaptersdealwiththetechnologyusedincurrentmole-
cularbiologyandbiotechnology.Thesedealprimarilywithcorenucleic
acidtechniquesandproteinexpressionthroughmicrobialandgenetic
detectionmethods.Furtherchaptersaddressthehugeadvancesmadein
geneandgenomeanalysisandupdatetherapidadvancesintoyeast
analysis,whichisprovidingveryexcitinginsightsintomolecularpath-
ways.Molecularbiologyalsocontinuestoaectprofoundlyprogressin
biotechnologyinareassuchasvaccinedevelopment,useandapplication
ofmonoclonalantibodies,clinicaltreatmentanddiagnosis,thepro-
ductionoftransgenicanimals,andmanyotherareasofresearchrelevant
tothepharmaceuticalindustry.Chaptersonalltheseareashavebeen
retainedandfullyupdatedinthisneweditionandnewchaptersintro-
ducedontheapplicationsofmolecularbiologyintheareasofdrug
designanddiseases,andregenerativemedicine.Inaddition,wecontinue
toensurethatbiotechnologyisnotjustconsideredatthegeneleveland
fullconsiderationcontinuestobegiventoapplicationsand
MolecularBiologyandBiotechnology,5thEdition
EditedbyJohnMWalkerandRalphRapley
r
RoyalSocietyofChemistry2009
PublishedbytheRoyalSocietyofChemistry,www.rsc.org
v
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